The Legionella pneumophila rpoS gene is required for growth within Acanthamoeba castellanii.

ABSTRACT: To investigate regulatory networks in Legionella pneumophila, the gene encoding the homolog of the Escherichia coli stress and stationary-phase sigma factor RpoS was identified by complementation of an E. coli rpoS mutation. An open reading frame that is approximately 60% identical to the E. coli rpoS gene was identified. Western blot analysis showed that the level of L. pneumophila RpoS increased in stationary phase. An insertion mutation was constructed in the rpoS gene on the chromosome of L. pneumophila, and the ability of this mutant strain to survive various stress conditions was assayed and compared with results for the wild-type strain. Both the mutant and wild-type strains were more resistant to stress when in stationary phase than when in the logarithmic phase of growth. This fin

SUBMITTER: Hales LM 

PROVIDER: S-EPMC93975 | biostudies-literature | 1999 Aug

REPOSITORIES: biostudies-literature