Development of a traditional Chinese medicine-based agent for the treatment of cancer cachexia.

ABSTRACT:

Background

Despite recent advances in understanding the pathophysiology of cancer cachexia, prevention/treatment of this debilitating disease remains an unmet medical need.

Methods

We developed an integrated, multi-tiered strategy involving both in vitro and in vivo muscle atrophy platforms to identify traditional Chinese medicine (TCM)-based anti-cachectic agents. In the initial screening, we used inflammatory cytokine-induced atrophy of C2C12 myotubes as a phenotypic screening platform to assess the protective effects of TCMs. The selected TCMs were then evaluated for their abilities to protect Caenorhabditis elegans from age-related reduction of mobility and contractility, followed by the C-26 colon adenocarcinoma mouse model of cachexia to confirm the anti-muscle atrophy

SUBMITTER: Wu KC 

PROVIDER: S-EPMC9397559 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature