Changes in physicochemical and anticancer properties modulated by chemically modified sugar moieties within sequence-related G-quadruplex structures.

ABSTRACT: We systematically investigated the influence of locked nucleic acid (LNA), unlock nucleic acid (UNA), and 2'-O-methyl-RNA (2'-O-Me-RNA) residues on the thermal stability, structure folding topology, biological activity and enzymatic resistance of three sequence-related DNA G-quadruplexes. In order to better understand the mechanism of action of the studied modifications, a single-position substitution in the loops or G-tetrads was performed and their influence was analyzed for a total of twenty-seven modified G-quadruplex variants. The studies show that the influence of each modification on the physicochemical properties of G-quadruplexes is position-dependent, due to mutual interactions between G-tetrads, loops, and additional guanosine at 5' or 3' end. Nevertheless, the anticancer activi

SUBMITTER: Roxo C 

PROVIDER: S-EPMC9397905 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature