Evaluation of Methods and Processes for Robust Monitoring of SARS-CoV-2 in Wastewater.

ABSTRACT: The SARS-CoV-2 pandemic has accelerated the development of virus concentration and molecular-based virus detection methods, monitoring systems and overall approach to epidemiology. Early into the pandemic, wastewater-based epidemiology started to be employed as a tool for tracking the virus transmission dynamics in a given area. The complexity of wastewater coupled with a lack of standardized methods led us to evaluate each step of the analysis individually and see which approach gave the most robust results for SARS-CoV-2 monitoring in wastewater. In this article, we present a step-by-step, retrospective view on the method development and implementation for the case of a pilot monitoring performed in Slovenia. We specifically address points regarding the thermal stability of the samples d

SUBMITTER: Maksimovic Carvalho Ferreira O 

PROVIDER: S-EPMC9398038 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature