Prediction and identification of epitopes in the Echinococcus multilocularis thrombospondin 3 antigen.

ABSTRACT:

Background

Alveolar echinococcosis is an epidemic disease caused by the parasitism of Echinococcus multilocularis (Em) larvae in the intermediate or final host.

Objective

To identify and analyze B-cell and T-cell (Th1, Th2, and Th17) epitopes of the Em antigen protein thrombospondin 3 (TSP3).

Methods

The amino acid sequence of TSP3 was obtained, and the secondary structural characteristics of TSP3 were predicted using bioinformatics software to further predict its potential T-cell and B-cell epitopes. The spleen lymphocytes of BALB/c mice, which were immunized with the TSP3 protein, were collected for co-culture with B-cell and T-cell antigen small peptides. The B-cell epitopes and T-cell epitope subtypes Th1, Th2, and Th17 were identified as having good immunogenicit

SUBMITTER: Pang MQ 

PROVIDER: S-EPMC9398089 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature