Whole-genome bisulfite sequencing analysis of circulating tumour DNA for the detection and molecular classification of cancer.

ABSTRACT:

Background

Cancer cell-specific variation and circulating tumour DNA (ctDNA) methylation are promising biomarkers for non-invasive cancer detection and molecular classification. Nevertheless, the applications of ctDNA to the early detection and screening of cancer remain highly challenging due to the scarcity of cancer cell-specific ctDNA, the low signal-to-noise ratio of DNA variation, and the lack of non-locus-specific DNA methylation technologies.

Methods

We enrolled three cohorts of breast cancer (BC) patients from two hospitals in China (BC: n = 123; healthy controls: n = 40). We developed a ctDNA whole-genome bisulfite sequencing technology employing robust trace ctDNA capture from up to 200 μL plasma, mini-input (1 ng) library preparation, unbiased genome-wide coverag

SUBMITTER: Gao Y 

PROVIDER: S-EPMC9398227 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature