CD8 T cell function and cross-reactivity explored by stepwise increased peptide-HLA versus TCR affinity.

ABSTRACT: Recruitment and activation of CD8 T cells occur through specific triggering of T cell receptor (TCR) by peptide-bound human leucocyte antigen (HLA) ligands. Within the generated trimeric TCR-peptide:HLA complex, the molecular binding affinities between peptide and HLA, and between TCR and peptide:HLA both impact T cell functional outcomes. However, how their individual and combined effects modulate immunogenicity and overall T cell responsiveness has not been investigated systematically. Here, we established two panels of human tumor peptide variants differing in their affinity to HLA. For precise characterization, we developed the "blue peptide assay", an upgraded cell-based approach to measure the peptide:HLA affinity. These peptide variants were then used to investigate the cross-reacti

SUBMITTER: Baumgaertner P 

PROVIDER: S-EPMC9399405 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature