Downregulation of miRNA miR-1305 and upregulation of miRNA miR-6785-5p may be associated with psoriasis.

ABSTRACT: Background: The role of serum extracellular vesicles (EVs) is less known in psoriasis. Objectives: To explore the transcriptomic profile of serum EVs and the potential biomarkers in psoriasis. Methods: EVs were isolated by differential ultracentrifugation and identified by transmission electron microscope. The diameters of EVs were detected using nanoparticle tracking analysis. Serum EVs-keratinocyte interaction was observed through confocal fluorescence microscopy. miRNA microarray and mRNA microarray were performed in serum EVs (n = 4) and skin lesions (n = 3), respectively. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) and fluorescence in situ hybridization were used to detect the expression of miRNAs in serum EVs and sk

SUBMITTER: Yan J 

PROVIDER: S-EPMC9399421 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature