Highly efficient and automated isolation technology for extracellular vesicles microRNA.

ABSTRACT: MicroRNA (miRNA) in extracellular vesicles (EVs) has great potential to be a promising marker in liquid biopsy. However, the present EV isolation methods, such as ultracentrifugation, have complicated and long-time operation, which impedes research on EV miRNA. The downstream complex miRNA extraction process will also significantly increase the detection cycle and loss. We first established a simple automated technique to efficiently extract target miRNAs in EVs from plasma based on Fe₃O₄@TiO₂ beads with high affinity and capture efficiency. We combined a heat-lysis method for quick and simple EV miRNA extraction and detection. The results indicated that our method has more RNA yield than TRIzol or a commercial kit and could complete EV enrichment and miRNA

SUBMITTER: Di K 

PROVIDER: S-EPMC9399425 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature