Application of MinION sequencing as a tool for the rapid detection and characterization of Listeria monocytogenes in smoked salmon.

ABSTRACT: Microbial pathogens may be present in different types of foods, and hence the development of novel methods to assure consumers' safeness is of great interest. Molecular methods are known to provide sensitive and rapid results; however, they are typically targeted approaches. In recent years, the advent of non-targeted approaches based on next-generation sequencing (NGS) has emerged as a rational way to proceed. This technology allows for the detection of several pathogens simultaneously. Furthermore, with the same set of data, it is possible to characterize the microorganisms in terms of serotype, virulence, and/ or resistance genes, among other molecular features. In the current study, a novel method for the detection of Listeria monocytogenes based on the "quasimetagenomics" appro

SUBMITTER: Azinheiro S 

PROVIDER: S-EPMC9399719 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature