Antiphospholipid antibodies are persistently positive at high titers. Additive value of platelet-bound C4d.

ABSTRACT:

Background

Classification criteria for antiphospholipid syndrome (APS) require that antiphospholipid antibody (aPL) positivity is confirmed after at least 12 weeks. We tested the hypothesis that aPL at high titers remain positive while low titers fluctuate over time. As both platelet-bound C4d (PC4d) and aPL are associated with thrombosis in systemic lupus erythematosus (SLE), we also evaluated whether PC4d can aid in APS diagnosis.

Methods

Data from serum or plasma sent to Exagen's laboratory for routine aPL testing were analyzed. Anti-cardiolipin (aCL) and anti-beta2 glycoprotein-1 antibodies (aB2GP1) were measured by chemiluminescence or ELiA fluorescence enzyme immunoassay; anti-phosphatidylserine/prothrombin complex antibodies (aPS/PT) by ELISA; PC4d by flow cytometry.

SUBMITTER: Sciascia S 

PROVIDER: S-EPMC9399821 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature