Cdc42 upregulation under high glucose induces podocyte apoptosis and impairs β-cell insulin secretion.

ABSTRACT:

Objectives

The progressive impairment of β-cell function results in prolonged deterioration in patients with type 2 diabetes mellitus (T2DM). Interestingly, the finding on pancreatitis secondary to renal injury suggests that potential communication exists between kidney and pancreas. Therefore, we aimed to investigate cell division cycle 42 (Cdc42)-mediated podocyte apoptosis and its effect on insulin secretion in islet β-cells.

Methods

Type 2 diabetic nephropathy mouse models were established to identify the expression of Cdc42 in podocytes by immunohistochemistry. An in vitro co-culture of mouse podocyte MPC5 and β-TC6 cells was preliminarily established. Subsequently, podocyte apoptosis induced by high glucose and Cdc42 was detected by TUNEL staining and western bl

SUBMITTER: Jiang S 

PROVIDER: S-EPMC9399854 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature