Differentiation of otitis media-causing bacteria and biofilms via Raman spectroscopy and optical coherence tomography.

ABSTRACT: In the management of otitis media (OM), identification of causative bacterial pathogens and knowledge of their biofilm formation can provide more targeted treatment approaches. Current clinical diagnostic methods rely on the visualization of the tympanic membrane and lack real-time assessment of the causative pathogen(s) and the nature of any biofilm that may reside behind the membrane and within the middle ear cavity. In recent years, optical coherence tomography (OCT) has been demonstrated as an improved in vivo diagnostic tool for visualization and morphological characterization of OM biofilms and middle ear effusions; but lacks specificity about the causative bacterial species. This study proposes the combination of OCT and Raman spectroscopy (RS) to examine differences in the r

SUBMITTER: Locke AK 

PROVIDER: S-EPMC9400059 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature