Light-Driven [FeFe] Hydrogenase Based H₂ Production in E. coli: A Model Reaction for Exploring E. coli Based Semiartificial Photosynthetic Systems.

ABSTRACT: Biohybrid technologies like semiartificial photosynthesis are attracting increased attention, as they enable the combination of highly efficient synthetic light-harvesters with the self-healing and outstanding performance of biocatalysis. However, such systems are intrinsically complex, with multiple interacting components. Herein, we explore a whole-cell photocatalytic system for hydrogen (H₂) gas production as a model system for semiartificial photosynthesis. The employed whole-cell photocatalytic system is based on Escherichia coli cells heterologously expressing a highly efficient, but oxygen-sensitive, [FeFe] hydrogenase. The system is driven by the organic photosensitizer eosin Y under broad-spectrum white light illumination. The direct involvement of the [FeFe] hyd

SUBMITTER: Lorenzi M 

PROVIDER: S-EPMC9400101 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature