Single-cell RNA sequencing profiling of mouse endothelial cells in response to pulmonary arterial hypertension.

ABSTRACT:

Aims

Endothelial cell (EC) dysfunction drives the initiation and pathogenesis of pulmonary arterial hypertension (PAH). We aimed to characterize EC dynamics in PAH at single-cell resolution.

Methods and results

We carried out single-cell RNA sequencing (scRNA-seq) of lung ECs isolated from an EC lineage-tracing mouse model in Control and SU5416/hypoxia-induced PAH conditions. EC populations corresponding to distinct lung vessel types, including two discrete capillary populations, were identified in both Control and PAH mice. Differential gene expression analysis revealed global PAH-induced EC changes that were confirmed by bulk RNA-seq. This included upregulation of the major histocompatibility complex class II pathway, supporting a role for ECs in the inflammatory response

SUBMITTER: Rodor J 

PROVIDER: S-EPMC9400412 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature