Colorimetric screening of elevated urinary mercury levels by a novel Hg²⁺-selective probe of resorufin phosphinothioate.

ABSTRACT: Urinary mercury levels are the most reliable indicators of mercury exposure but identifying them requires complex techniques and heavy instruments. In this research, we reported a simple and convenient urinary mercury analysis method using a readily available office scanner. Probe MP-1 synthesized by the reaction of resorufin and dimethylthiophosphinoyl chloride revealed Hg²⁺-selective chromogenic and fluorescent signaling behavior. Signaling was realized through Hg²⁺-induced deprotection of the phosphinothioate protecting group in the resorufin-based probe MP-1 to yield the parent fluorochrome. A pronounced colorimetric response of color change from light yellow to pink alongside a turn-on type fluorescence enhancement was perceived exclusively toward Hg²⁺

SUBMITTER: Choi MG 

PROVIDER: S-EPMC9400507 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature