Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates.

ABSTRACT:

Aim

Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted.

Methods

Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC), and differential scanning calorimetry (DSC)] were tested for this comparison.

Results

Maytansinoid-based ADCs showed less hydrophobicity than auristatin-based ADCs. Regardless of the drug-linker and drug-to-antibody ratios (DARs), the stability detected by DSC was decreased by conjugation.

Conclusions

The cost and time-efficient analytical comparison described in this manuscript may be useful information for an initial characterization of ADCs prior to detailed biol

SUBMITTER: Fujii T 

PROVIDER: S-EPMC9400747 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature