Unravelling the Turn-On Fluorescence Mechanism of a Fluorescein-Based Probe in GABA_A Receptors.

ABSTRACT: GABA_A (γ-aminobutyric acid type A) receptors are ligand-gated ion channels mediating fast inhibitory transmission in the mammalian brain. Here we report the molecular and electronic mechanism governing the turn-on emission of a fluorescein-based imaging probe able to target the human GABA_A receptor. Multiscale calculations evidence a drastic conformational change of the probe from folded in solution to extended upon binding to the receptor. Intramolecular ππ-stacking interactions present in the folded probe are responsible for quenching fluorescence in solution. In contrast, unfolding within the GABA_A receptor changes the nature of the bright excited state triggering emission. Remarkably, this turn-on effect only manifests for the dianionic prototropic form

SUBMITTER: Singer NK 

PROVIDER: S-EPMC9400872 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature