Spectroscopic and in vitro Investigations of Fe²⁺ /α-Ketoglutarate-Dependent Enzymes Involved in Nucleic Acid Repair and Modification.

ABSTRACT: The activation of molecular oxygen for the highly selective functionalization and repair of DNA and RNA nucleobases is achieved by α-ketoglutarate (α-KG)/iron-dependent dioxygenases. Of special interest are the human homologues AlkBH of Escherichia coli EcAlkB and ten-eleven translocation (TET) enzymes. These enzymes are involved in demethylation or dealkylation of DNA and RNA, although additional physiological functions are continuously being found. Given their importance, studying enzyme-substrate interactions, turnover and kinetic parameters is pivotal for the understanding of the mode of action of these enzymes. Diverse analytical methods, including X-ray crystallography, UV/Vis absorption, electron paramagnetic resonance (EPR), circular dichroism (CD) and nuclear magnetic resonance (N

SUBMITTER: Schmidl D 

PROVIDER: S-EPMC9401043 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature