High Versatility of IPP and DMAPP Methyltransferases Enables Synthesis of C₆ , C₇ and C₈ Terpenoid Building Blocks.

ABSTRACT: The natural substance class of terpenoids covers an extremely wide range of different structures, although their building block repertoire is limited to the C₅ compounds DMAPP and IPP. This study aims at the characterization of methyltransferases (MTases) that modify these terpene precursors and the demonstration of their suitability for biotechnological purposes. All seven enzymes tested accepted IPP as substrate and altogether five C₆ compounds and six C₇ compounds were formed within the reactions. A high selectivity for the deprotonation site as well as high stereoselectivity could be observed for most of the biocatalysts. Only the enzyme from Micromonospora humi also accepted DMAPP as substrate, converting it into (2R)-2-methyl-IPP in vitro. In vivo stu

SUBMITTER: Drummond L 

PROVIDER: S-EPMC9401056 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature