HPV Sequencing Facilitates Ultrasensitive Detection of HPV Circulating Tumor DNA.

ABSTRACT:

Purpose

Human papillomavirus (HPV) DNA offers a convenient circulating tumor DNA (ctDNA) marker for HPV-associated malignancies, but current methods, such as digital PCR (dPCR), provide insufficient accuracy for clinical applications in patients with low disease burden. We asked whether a next-generation sequencing approach, HPV sequencing (HPV-seq), could provide quantitative and qualitative assessment of HPV ctDNA in low disease burden settings.

Experimental design

We conducted preclinical technical validation studies on HPV-seq and applied it retrospectively to a prospective multicenter cohort of patients with locally advanced cervix cancer (NCT02388698) and a cohort of patients with oropharynx cancer. HPV-seq results were compared with dPCR. The primary outcome was progr

SUBMITTER: Leung E 

PROVIDER: S-EPMC9401563 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature