Reconstitution of a telomeric replicon organized by CST.

ABSTRACT: Telomeres, the natural ends of linear chromosomes, comprise repeat-sequence DNA and associated proteins¹. Replication of telomeres allows continued proliferation of human stem cells and immortality of cancer cells². This replication requires telomerase³ extension of the single-stranded DNA (ssDNA) of the telomeric G-strand ((TTAGGG)_n); the synthesis of the complementary C-strand ((CCCTAA)_n) is much less well characterized. The CST (CTC1-STN1-TEN1) protein complex, a DNA polymerase α-primase accessory factor^4,5, is known to be required for telomere replication in vivo^6-9, and the molecular analysis presented here reveals key features of its mechanism. We find that human CST uses its ssDNA-binding activity to specif

SUBMITTER: Zaug AJ 

PROVIDER: S-EPMC9402439 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature