Diverse mutational landscapes in human lymphocytes.

ABSTRACT: The lymphocyte genome is prone to many threats, including programmed mutation during differentiation¹, antigen-driven proliferation and residency in diverse microenvironments. Here, after developing protocols for expansion of single-cell lymphocyte cultures, we sequenced whole genomes from 717 normal naive and memory B and T cells and haematopoietic stem cells. All lymphocyte subsets carried more point mutations and structural variants than haematopoietic stem cells, with higher burdens in memory cells than in naive cells, and with T cells accumulating mutations at a higher rate throughout life. Off-target effects of immunological diversification accounted for approximately half of the additional differentiation-associated mutations in lymphocytes. Memory B cells acquired, on av

SUBMITTER: Machado HE 

PROVIDER: S-EPMC9402440 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature