HMMR promotes peritoneal implantation of gastric cancer by increasing cell-cell interactions.

ABSTRACT:

Background

Distant metastasis is the prominent factor for cancer-induced death of gastric cancer in which peritoneum is one of the dominating targets of gastric cancer metastasis. However, there is still a lack of effective predictive indicators and treatment methods for gastric cancer patients with peritoneal metastasis.

Methods

A clustering assay was used to investigate the cell aggregates formation ability. While the soft agar assay and anoikis assay were performed to detect the anchorage-independent growth and anoikis-resistant ability respectively. Luciferase activity assay, western blotting and immunofluorescence were used to explore the effect of HMMR on AKT signaling activity. The peritoneal implantation model was examined to explore the role of HMMR in vivo.

Resu

SUBMITTER: Yang M 

PROVIDER: S-EPMC9402864 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature