An Automated Approach to Assess Relative Galectin-Glycan Affinity Following Glycan Microarray Analysis.

ABSTRACT: Numerous studies have highlighted the utility of glycan microarray analysis for the elucidation of protein-glycan interactions. However, most current glycan microarray studies analyze glycan binding protein (GBP)-glycan interactions at a single protein concentration. While this approach provides useful information related to a GBP's overall binding capabilities, extrapolation of true glycan binding preferences using this method fails to account for printing variations or other factors that may confound relative binding. To overcome this limitation, we examined glycan array binding of three galectins over a range of concentrations to allow for a more complete assessment of binding preferences. This approach produced a richer data set than single concentration analysis and provided more accu

SUBMITTER: Ho AD 

PROVIDER: S-EPMC9403319 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature