Global signalling network analysis of luminal T47D breast cancer cells in response to progesterone.

ABSTRACT:

Background

Breast cancer cells enter into the cell cycle following progestin exposure by the activation of signalling cascades involving a plethora of enzymes, transcription factors and co-factors that transmit the external signal from the cell membrane to chromatin, ultimately leading to a change of the gene expression program. Although many of the events within the signalling network have been described in isolation, how they globally team up to generate the final cell response is unclear.

Methods

In this study we used antibody microarrays and phosphoproteomics to reveal a dynamic global signalling map that reveals new key regulated proteins and phosphor-sites and links between previously known and novel pathways. T47D breast cancer cells were used, and phospho-sites and p

SUBMITTER: Wright RHG 

PROVIDER: S-EPMC9403329 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature