Conversion of hepatitis B virus relaxed circular to covalently closed circular DNA is supported in murine cells

ABSTRACT:

Background & Aims

HBV has a narrow host restriction, with humans and chimpanzees representing the only known natural hosts. The molecular correlates of resistance in species that are commonly used in biomedical research, such as mice, are currently incompletely understood. Expression of human NTCP (hNTCP) in mouse hepatocytes enables HBV entry, but subsequently covalently closed circular (cccDNA) does not form in most murine cells. It is unknown if this blockade in cccDNA formation is due to deficiency in repair of relaxed circular DNA (rcDNA) to cccDNA.

Methods

Here, we deployed both in vivo and in vitro virological and biochemical approaches to investigate if murine cells contain a complete set of repair factors capable of converting HBV rcDNA to cccDNA.

Results

SUBMITTER: Wei L 

PROVIDER: S-EPMC9403495 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature