Molecular clones of genetically distinct hepatitis B virus genotypes reveal distinct host and drug treatment responses.

ABSTRACT:

Background & aims

HBV exhibits wide genetic diversity with at least 9 genotypes (GTs), which differ in terms of prevalence, geographic distribution, natural history, disease progression, and treatment outcome. However, differences in HBV replicative capacity, gene expression, and infective capability across different GTs remain incompletely understood. Herein, we aimed to study these crucial aspects using newly constructed infectious clones covering the major HBV GTs.

Methods

The replicative capacity of infectious clones covering HBV GTs A-E was analyzed in cell lines, primary hepatocytes and humanized mice. Host responses and histopathology induced by the different HBV GTs were characterized in hydrodynamically injected mice. Differences in treatment responses to entecavir

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9403497 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature