Reagentless electrochemical biosensors through incorporation of unnatural amino acids on the protein structure.

ABSTRACT: Typical protein biosensors employ chemical or genetic labeling of the protein, thus introducing an extraneous molecule to the wild-type parent protein, often changing the overall structure and properties of the protein. While these labeling methods have proven successful in many cases, they also have a series of disadvantages associated with their preparation and function. An alternative route for labeling proteins is the incorporation of unnatural amino acid (UAA) analogues, capable of acting as a label, into the structure of a protein. Such an approach, while changing the local microenvironment, poses less of a burden on the overall structure of the protein. L-DOPA is an analog of phenylalanine and contains a catechol moiety that participates in a quasi-reversible, two-electron redox pro

SUBMITTER: Zeynaloo E 

PROVIDER: S-EPMC9404255 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature