DNA-Based Smart Reagent for Detecting Alzheimer's Associated MicroRNAs.

ABSTRACT: Alzheimer's disease (AD) is the most common neurodegenerative disorder, with significant research efforts devoted to identifying new biomarkers for clinical diagnosis and treatment. MicroRNAs have emerged as likely disease regulators and biomarkers for AD, now implicated as having roles in several biological processes related to progression of the disease. In this work, we use the miRacles assay (microRNA activated conditional looping of engineered switches) for single-step detection of AD-related microRNAs. The technology is based on conformationally responsive DNA nanoswitches that loop upon recognition of a target microRNA and report their on/off status through an electrophoretic readout. Unlike many methods, our approach directly

SUBMITTER: Chandrasekaran AR 

PROVIDER: S-EPMC9404293 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature