Both T and B cells are indispensable for the development of a PBMC transfer-induced humanized mouse model for SSc.

ABSTRACT:

Background

Recently, a novel humanized mouse model for systemic sclerosis (SSc) was established by transferring peripheral blood mononuclear cells (PBMC) from patients with SSc to Rag2^-/-Il2rg^-/- immunodeficient mice. Here, we aimed to investigate the role of T and B cells in this humanized mouse model.

Methods

T and B cells were depleted in vitro from freshly isolated PBMC using anti-CD3 and anti-CD19 magnetic microbeads, respectively. Subsequently, PBMC and T or B cell-depleted PBMC were transferred into Rag2^-/-/Il2rg^-/- mice via intraperitoneal injection. Twelve weeks after the transfer, mice were sacrificed and evaluated.

Results

Mice transferred with whole PBMC from SSc patients developed systemic inflammation in the lu

SUBMITTER: Shu Y 

PROVIDER: S-EPMC9404611 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature