Automated Computer-Assisted Image Analysis for the Fast Quantification of Kidney Fibrosis.

ABSTRACT: Chronic kidney disease (CKD) is a common and worldwide health problem and one of the most important causes of morbidity and mortality. Most primary research on this disease requires evaluating the fibrosis index in animal model kidneys, specifically using Masson's trichrome stain. Different programs are used to calculate the percentage of fibrosis; however, the analysis is time-consuming since one image must be performed at a time. CellProfiler™ is a program designed to analyze data obtained from biological samples and can process multiple images through pipelines, and the results can be exported to databases. This article explains how CellProfiler™ can be used to automatically analyze kidney histology photomicrographs from samples stained with Masson's trichrome stain to assess the percen

SUBMITTER: Sanchez-Jaramillo EA 

PROVIDER: S-EPMC9404825 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature