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Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue.

ABSTRACT: An imaging technique across multiple spatial scales is required for extracting structural information on neurons with processes of meter scale length and specialized nanoscale structures. Here, we present a protocol combining multi-scale light microscopy (LM) with electron microscopy (EM) in mouse brain tissue. We describe tissue slice preparation and LM/EM dual labeling with EGFP-APEX2 fusion protein. We then detail ScaleSF tissue clearing and successive LM/EM imaging. Our protocol allows for deciphering structural information across multiple spatial scales on neurons. For complete details on the use and execution of this protocol, please refer to Furuta et al. (2022).

SUBMITTER: Yamauchi K

PROVIDER: S-EPMC9405099 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue.

Yamauchi Kenta K Furuta Takahiro T Okamoto Shinichiro S Takahashi Megumu M Koike Masato M Hioki Hiroyuki H

STAR protocols 20220818 3

An imaging technique across multiple spatial scales is required for extracting structural information on neurons with processes of meter scale length and specialized nanoscale structures. Here, we present a protocol combining multi-scale light microscopy (LM) with electron microscopy (EM) in mouse brain tissue. We describe tissue slice preparation and LM/EM dual labeling with EGFP-APEX2 fusion protein. We then detail Sca<i>l</i>eSF tissue clearing and successive LM/EM imaging. Our protocol allow ...[more]

PMID: 36035789

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