Multicellular Cell Seeding on a Chip: New Design and Optimization towards Commercialization.

ABSTRACT: This paper shows both experimental and in-depth theoretical studies (including simulations and analytical solutions) on a microfluidic platform to optimize its design and use for 3D multicellular co-culture applications, e.g., creating a tissue-on-chip model for investigating diseases such as pulmonary arterial hypertension (PAH). A tissue microfluidic chip usually has more than two channels to seed cells and supply media. These channels are often separated by barriers made of micro-posts. The optimization for the structures of these micro-posts and their spacing distances is not considered previously, especially for the aspects of rapid and cost-efficient fabrication toward scaling up and commercialization. Our experimental and theoretical (COMSOL simulations and analytical solutions) res

SUBMITTER: Nguyen T 

PROVIDER: S-EPMC9405756 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature