Untargeted Multimodal Metabolomics Investigation of the Haemonchus contortus Exsheathment Secretome.

ABSTRACT: In nematodes that invade the gastro-intestinal tract of the ruminant, the process of larval exsheathment marks the transition from the free-living to the parasitic stages of these parasites. To investigate the secretome associated with larval exsheathment, a closed in vitro system that effectively reproduces the two basic components of an anaerobic rumen environment (CO₂ and 39 °C) was developed to trigger exsheathment in one of the most pathogenic and model gastrointestinal parasitic nematodes, Haemonchus contortus (barber's pole worm). This study reports the use of multimodal untargeted metabolomics and lipidomics methodologies to identify the metabolic signatures and compounds secreted during in vitro larval exsheathment in the H. contortus infective third-stage

SUBMITTER: Palevich N 

PROVIDER: S-EPMC9406637 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature