Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads.

ABSTRACT: The complex and lengthy protocol of current viral nucleic acid extraction processes limits their use outside laboratory settings. Here, we describe a rapid and reliable method for extracting nucleic acids from viral samples using a rotating blade and magnetic beads. The viral membrane can be instantly lysed using a high-speed rotating blade, and nucleic acids can be immediately isolated using a silica magnetic surface. The process was completed within 60 s by this method. Routine washing and eluting processes were subsequently conducted within 5 min. The results achieved by this method were comparable to those of a commercially available method. When the blade-based lysis and magnetic bead adsorption processes were performed separately, the RNA recovery rate was very low, and the Ct value

SUBMITTER: Bae M 

PROVIDER: S-EPMC9407373 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature