Dataset Information

Elucidation of the Flavor Aspects and Flavor-Associated Genomic Regions in Bottle Gourd (Lagenaria siceraria) by Metabolomic Analysis and QTL-seq.

ABSTRACT: Bottle gourd (Lagenaria siceraria) is a commercially important cucurbitaceous vegetable with health-promoting properties whose collections and cultivars differ considerably in their flavor aspects. However, the metabolomic profile related to flavor has not yet been elucidated. In the present study, a comprehensive metabolite analysis revealed the metabolite profile of the strong-flavor collection "J120" and weak-flavor collection "G32". The major differentially expressed metabolites included carboxylic acids, their derivatives, and organooxygen compounds, which are involved in amino acid biosynthesis and metabolism. QTL-seq was used to identify candidate genomic regions controlling flavor in a MAGIC population comprising 377 elite lines. Three significant genomic regions were identified, and candidate genes likely associated with flavor were screened. Our study provides useful information for understanding the metabolic causes of flavor variation among bottle gourd collections and cultivars. Furthermore, the identified candidate genomic regions may facilitate rational breeding programs to improve bottle gourd quality.

SUBMITTER: Wang Y

PROVIDER: S-EPMC9407550 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Publications

Elucidation of the Flavor Aspects and Flavor-Associated Genomic Regions in Bottle Gourd (<i>Lagenaria siceraria</i>) by Metabolomic Analysis and QTL-seq.

Wang Ying Y Li Yanwei Y Wu Xiaohua X Wu Xinyi X Feng Zishan Z Wang Jian J Wang Baogen B Lu Zhongfu Z Li Guojing G

Foods (Basel, Switzerland) 20220814 16

Bottle gourd (<i>Lagenaria siceraria</i>) is a commercially important cucurbitaceous vegetable with health-promoting properties whose collections and cultivars differ considerably in their flavor aspects. However, the metabolomic profile related to flavor has not yet been elucidated. In the present study, a comprehensive metabolite analysis revealed the metabolite profile of the strong-flavor collection "J120" and weak-flavor collection "G32". The major differentially expressed metabolites inclu ...[more]

PMID: 36010450

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Project description:BackgroundThe cysteine-rich receptor-like kinases (CRKs) family in plants have been reported to perform multiple functions against various stresses. However, the CRK family in bottle gourd (Lagenaria siceraria) has not been well-explored. Herein, a comprehensive genome-wide identification and characterization of the CRK gene family has been carried out in bottle gourd under Genome-wide characterization of CRK genes in bottle gourds under Fusarium oxysporum f. sp. lagenariae infection.ResultsA stringent set of bioinformatic analyses identified 18 LsCRKs in the bottle gourd genome. Chromosomal mapping of the identified LsCRKs revealed that the LsCRKs were distributed in 4 chromosomes in the bottle gourd genome. The phylogenetic analysis of LsCRKs divided them into two subgroups on the tree. The synteny and collinearity analysis of the LsCRKs among themselves and other plant CRKs provided insights into their conservancy and expansion. Gene ontology analysis of the identified LsCRKs suggested their possible roles in regulating different physiological processes and stress responses in bottle gourd. To assess the involvement of the LsCRKs under F. oxysporum f. sp. lagenariae infection, bottle gourd seedlings were transplanted into the pots with F. oxysporum-infected soil. The expression analysis revealed that multiple LsCRKs exhibited induced expression, suggesting their involvement in bottle gourd-F. oxysporum interactions. Additionally, the protein-protein interaction analysis suggested some important interacting partners of LsCRKs crucial to different physiological processes in bottle gourd.ConclusionsThe present work explored and analyzed the LsCRKs in bottle gourd. Functional predictions and interaction network analysis suggested the roles of LsCRKs in modulating multiple physiological processes in bottle gourd. The expression dynamics of LsCKRs under fungal pathogen infection suggest their involvement in stress response in bottle gourds. Overall, the results of the study provide basic information about the CRK family in bottle gourds and their involvement in fungal pathogen response.

Dataset Information

Elucidation of the Flavor Aspects and Flavor-Associated Genomic Regions in Bottle Gourd (Lagenaria siceraria) by Metabolomic Analysis and QTL-seq.

Publications

Elucidation of the Flavor Aspects and Flavor-Associated Genomic Regions in Bottle Gourd (<i>Lagenaria siceraria</i>) by Metabolomic Analysis and QTL-seq.

Similar Datasets

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