Examining atherosclerotic lesions in three dimensions at the nanometer scale with cryo-FIB-SEM.

ABSTRACT: We employed in a correlative manner an unconventional combination of methods, comprising cathodoluminescence, cryo-scanning electron microscopy (SEM), and cryo-focused ion beam (FIB)-SEM, to examine the volumes of thousands of cubed micrometers from rabbit atherosclerotic tissues, maintained in close-to-native conditions, with a resolution of tens of nanometers. Data from three different intralesional regions, at the media-lesion interface, in the core, and toward the lumen, were analyzed following segmentation and volume or surface representation. The media-lesion interface region is rich in cells and lipid droplets, whereas the core region is markedly richer in crystals and has lower cell density. In the three regions, thin crystals appear to be associated with intracellular or extracell

SUBMITTER: Capua-Shenkar J 

PROVIDER: S-EPMC9407640 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature