Efficient Base-Catalyzed Kemp Elimination in an Engineered Ancestral Enzyme.

ABSTRACT: The routine generation of enzymes with completely new active sites is a major unsolved problem in protein engineering. Advances in this field have thus far been modest, perhaps due, at least in part, to the widespread use of modern natural proteins as scaffolds for de novo engineering. Most modern proteins are highly evolved and specialized and, consequently, difficult to repurpose for completely new functionalities. Conceivably, resurrected ancestral proteins with the biophysical properties that promote evolvability, such as high stability and conformational diversity, could provide better scaffolds for de novo enzyme generation. Kemp elimination, a non-natural reaction that provides a simple model of proton abstraction from carbon, has been extensively used as a benchmark in de novo enzy

SUBMITTER: Gutierrez-Rus LI 

PROVIDER: S-EPMC9408544 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature