The Role of CD28 and CD8⁺ T Cells in Keloid Development.

ABSTRACT: Background: A keloid is a benign skin tumor that extends beyond the initial injury area, and its pathologic mechanism remains unclear. Method: High-throughput sequencing data were obtained from normal skin tissue of patients with keloids (Group N) and healthy controls (Group C). Important genes were mined by bioinformatics analysis and identified by RT-qPCR, Western blotting, immunohistochemistry and immunofluorescence assays. The CIBERSORT algorithm was used to convert gene expression information into immune cell information. Flow cytometry was used to verify the key immune cells. Fluorescence-activated cell sorting coculture and CCK8 experiments were used to explore the effect of CD8⁺ T cells on keloid-associated fibroblasts. Neural network models were used to co

SUBMITTER: Shan M 

PROVIDER: S-EPMC9408754 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature