Cell-Type Dependent Regulation of the Electrogenic Na⁺/HCO₃^- Cotransporter 1 (NBCe1) by Hypoxia and Acidosis in Glioblastoma.

ABSTRACT: Glioblastoma multiforme (GBM) is the most common and malignant brain tumour. It is characterised by transcriptionally distinct cell populations. In tumour cells, physiological pH gradients between the intracellular and extracellular compartments are reversed, compared to non-cancer cells. Intracellular pH in tumour cells is alkaline, whereas extracellular pH is acidic. Consequently, the function and/or expression of pH regulating transporters might be altered. Here, we investigated protein expression and regulation of the electrogenic sodium/bicarbonate cotransporter 1 (NBCe1) in mesenchymal (MES)-like hypoxia-dependent and -independent cells, as well as in astrocyte-like glioblastoma cells following chemical hypoxia, acidosis and elucidated putative underlying molecular pathways. Immunobl

SUBMITTER: Giannaki M 

PROVIDER: S-EPMC9408864 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature