Essential Roles of the Histone Demethylase KDM4C in Renal Development and Acute Kidney Injury.

ABSTRACT:

Background

Lysine demethylase 4C (KDM4C) is a nuclear protein that is essential for histone modification and acts as an important regulator of several transcription factors. Previous studies have shown that KDM4C may also play a role in mediating stress responses. The purpose of this study was to examine the roles of KDM4C in kidney development and acute kidney injury (AKI).

Methods

The effect of KDM4C on kidney development was assessed by comparing the kidney phenotype between 96 zebrafish embryos treated with kdm4c-morpholino oligonucleotide and 96 untreated zebrafish embryos. We further examined whether KDM4C is essential for maintaining cell survival in AKI. Cultured human renal tubular cells were used for the in vitro study. Wild-type and Kdm4c knockout mice (C57BL/6NTa

SUBMITTER: Pan HC 

PROVIDER: S-EPMC9409075 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature