Dataset Information

Magnetic-Responsive Doxorubicin-Containing Materials Based on Fe₃O₄ Nanoparticles with a SiO₂/PEG Shell and Study of Their Effects on Cancer Cell Lines.

ABSTRACT: Novel nanocomposite materials based on Fe₃O₄ magnetic nanoparticles (MNPs) coated with silica and covalently modified by [(3-triethoxysilyl)propyl]succinic acid-polyethylene glycol (PEG 3000) conjugate, which provides a high level of doxorubicin (Dox) loading, were obtained. The efficiency of Dox desorption from the surface of nanomaterials under the action of an alternating magnetic field (AMF) in acidic and neutral media was evaluated. Their high cytotoxicity against tumor cells, as well as the drug release upon application of AMF, which leads to an increase in the cytotoxic effect, was demonstrated.

SUBMITTER: Demin AM

PROVIDER: S-EPMC9409415 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Publications

Magnetic-Responsive Doxorubicin-Containing Materials Based on Fe3O4 Nanoparticles with a SiO2/PEG Shell and Study of Their Effects on Cancer Cell Lines.

Demin Alexander M AM Vakhrushev Alexander V AV Pershina Alexandra G AG Valova Marina S MS Efimova Lina V LV Syomchina Alexandra A AA Uimin Mikhail A MA Minin Artem S AS Levit Galina L GL Krasnov Victor P VP Charushin Valery N VN

International journal of molecular sciences 20220813 16

Novel nanocomposite materials based on Fe3O4 magnetic nanoparticles (MNPs) coated with silica and covalently modified by [(3-triethoxysilyl)propyl]succinic acid-polyethylene glycol (PEG 3000) conjugate, which provides a high level of doxorubicin (Dox) loading, were obtained. The efficiency of Dox desorption from the surface of nanomaterials under the action of an alternating magnetic field (AMF) in acidic and neutral media was evaluated. Their high cytotoxicity against tumo ...[more]

PMID: 36012356

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Project description:BackgroundUnderstanding the in vivo size-dependent pharmacokinetics and toxicity of nanoparticles is crucial to determine their successful development. Systematic studies on the size-dependent biological effects of nanoparticles not only help to unravel unknown toxicological mechanism but also contribute to the possible biological applications of nanomaterial.MethodsIn this study, the biodistribution and the size-dependent biological effects of Fe3O4@SiO2-NH2 nanoparticles (Fe@Si-NPs) in three diameters (10, 20 and 40 nm) were investigated by ICP-AES, serum biochemistry analysis and NMR-based metabolomic analysis after intravenous administration in a rat model.ResultsOur findings indicated that biodistribution and biological activities of Fe@Si-NPs demonstrated the obvious size-dependent and tissue-specific effects. Spleen and liver are the target tissues of Fe@Si-NPs, and 20 nm of Fe@Si-NPs showed a possible longer blood circulation time. Quantitative biochemical analysis showed that the alterations of lactate dehydrogenase (LDH) and uric acid (UA) were correlated to some extent with the sizes of Fe@Si-NPs. The untargeted metabolomic analyses of tissue metabolomes (kidney, liver, lung, and spleen) indicated that different sizes of Fe@Si-NPs were involved in the different biochemical mechanisms. LDH, formate, uric acid, and GSH related metabolites were suggested as sensitive indicators for the size-dependent toxic effects of Fe@Si-NPs. The findings from serum biochemical analysis and metabolomic analysis corroborate each other. Thus we proposed a toxicity hypothesis that size-dependent NAD depletion may occur in vivo in response to nanoparticle exposure. To our knowledge, this is the first report that links size-dependent biological effects of nanoparticles with in vivo NAD depletion in rats.ConclusionThe integrated metabolomic approach is an effective tool to understand physiological responses to the size-specific properties of nanoparticles. Our results can provide a direction for the future biological applications of Fe@Si-NPs.

Dataset Information

Magnetic-Responsive Doxorubicin-Containing Materials Based on Fe₃O₄ Nanoparticles with a SiO₂/PEG Shell and Study of Their Effects on Cancer Cell Lines.

Publications

Magnetic-Responsive Doxorubicin-Containing Materials Based on Fe<sub>3</sub>O<sub>4</sub> Nanoparticles with a SiO<sub>2</sub>/PEG Shell and Study of Their Effects on Cancer Cell Lines.

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