Conformational Variability in Ground-State CFTR Lipoprotein Particle Cryo-EM Ensembles.

ABSTRACT: Cystic fibrosis transmembrane regulator (CFTR) is a dynamic membrane protein belonging to the ABC transporter family. It is unusual within this family as it is an ion channel, as opposed to a transporter. Activation of CFTR requires ATP and phosphorylation by PKA, and dysregulation of CFTR mediated salt and water homeostasis can lead to cystic fibrosis. Recent advancements in structural biological methods have led to more than 10 published CFTR structures, and, so far, all of these structures of CFTR, determined by cryo-EM, have been limited to detergent-purified protein preparations. To visualize CFTR in an environment that more closely represents its native membranous environment, we utilized two different lipoprotein particle encapsulation techniques: one in which the ion channel is fir

SUBMITTER: Aleksandrov LA 

PROVIDER: S-EPMC9409475 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature