Reference Genes Selection of Gymnosporangium yamadae during the Interaction with Apple Leaves.

ABSTRACT: Apple rust disease caused by Gymnosporangium yamadae is the one of the major threats to the development of the apple industry in China, but the pathogenic molecular mechanism of the disease remains unclear. It is imperative to screen out appropriate reference genes during the interaction between G. yamadae and apple leaves to analyze the gene expression patterns during the pathogenesis of G. yamadae. ACT, EF1, EF2, GAPDH, 40S, 60S, α-TUB, β-TUB and UBCE3 were selected as candidate reference genes based on the transcriptomic dataset of G. yamadae. The expression levels were tested by real-time quantitative PCR during time-course infection of apple leaves and the expression stabilities were evaluated b

SUBMITTER: Shao C 

PROVIDER: S-EPMC9409963 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature