Novel 3D-Printed Cell Culture Inserts for Air-Liquid Interface Cell Culture.

ABSTRACT: In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of '3Rs', new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inserts to culture SE are commercially available, however, these inserts are expensive and of limited versatility regarding experimental settings. This study aimed to design novel cell culture inserts fabricated on commercially available 3D printers for the generation of full-thickness SE. A computer-aided design model was realized by extrusion-based 3D printing of polylactic acid filaments (PLA). Improvements in the design of the inserts for easier and more efficient handling were confirmed in cell

SUBMITTER: Bauer M 

PROVIDER: S-EPMC9410432 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature