Evolution of β-lactamase-mediated cefiderocol resistance.

ABSTRACT:

Background

Cefiderocol is a novel siderophore β-lactam with improved hydrolytic stability toward β-lactamases, including carbapenemases, achieved by combining structural moieties of two clinically efficient cephalosporins, ceftazidime and cefepime. Consequently, cefiderocol represents a treatment alternative for infections caused by MDR Gram-negatives.

Objectives

To study the role of cefiderocol on resistance development and on the evolution of β-lactamases from all Ambler classes, including KPC-2, CTX-M-15, NDM-1, CMY-2 and OXA-48.

Methods

Directed evolution, using error-prone PCR followed by selective plating, was utilized to investigate how the production and the evolution of different β-lactamases cause changes in cefiderocol susceptibility determined using microb

SUBMITTER: Frohlich C 

PROVIDER: S-EPMC9410664 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature