Development of a genome-wide marker design workflow for onions and its application in target amplicon sequencing-based genotyping.

ABSTRACT: Onions are one of the most widely cultivated vegetables worldwide; however, the development and utilization of molecular markers have been limited because of the large genome of this plant. We present a genome-wide marker design workflow for onions and its application in a high-throughput genotyping method based on target amplicon sequencing. The efficiency of the method was evaluated by genotyping of F2 populations. In the marker design workflow, unigene and genomic sequence data sets were constructed, and polymorphisms between parental lines were detected through transcriptome sequence analysis. The positions of polymorphisms detected in the unigenes were mapped onto the genome sequence, and primer sets were designed. In total, 480 markers covering the whole genome were selected. By geno

SUBMITTER: Sekine D 

PROVIDER: S-EPMC9410872 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature