The expression of aminoglycoside resistance genes in integron cassettes is not controlled by riboswitches.

ABSTRACT: Regulation of gene expression is a key factor influencing the success of antimicrobial resistance determinants. A variety of determinants conferring resistance against aminoglycosides (Ag) are commonly found in clinically relevant bacteria, but whether their expression is regulated or not is controversial. The expression of several Ag resistance genes has been reported to be controlled by a riboswitch mechanism encoded in a conserved sequence. Yet this sequence corresponds to the integration site of an integron, a genetic platform that recruits genes of different functions, making the presence of such a riboswitch counterintuitive. We provide, for the first time, experimental evidence against the existence of such Ag-sensing riboswitch. We first tried to reproduce the induction of the well

SUBMITTER: Hipolito A 

PROVIDER: S-EPMC9410878 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature