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SARS-CoV-2 CD8⁺ T cell killing assays using replicating viruses and transgenic antigens.

ABSTRACT: The quality of an antigen-specific CD8⁺ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8⁺ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For complete details on the use and execution of this protocol, please refer to Moosmann et al. (2022) and Wagner et al. (2022).

SUBMITTER: Mateyka LM

PROVIDER: S-EPMC9411149 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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SARS-CoV-2 CD8+ T cell killing assays using replicating viruses and transgenic antigens.

Mateyka Laura M LM Grass Vincent V Pichlmair Andreas A Busch Dirk H DH D'Ippolito Elvira E

STAR protocols 20220826 4

The quality of an antigen-specific CD8+ T cell repertoire is crucial for the clearance of intracellular pathogens, in particular for viral infections. Here, we describe killing assays to determine the function of CD8+ T cells engineered with SARS-CoV-2-specific T cell receptors in a near-physiological system for antigen presentation. We detail the use of target cells either infected with replicating SARS-CoV-2 virus or engineered with SARS-CoV-2 open reading frames. For com ...[more]

PMID: 36152303

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